In vitro hydroxyapatite formation of a tetracalcium phosphate and anhydrous dicalcium phosphate based dentine desensitiser: TRIS buffer vs artificial saliva.


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Published: Aug 1, 2021
DOI: https://doi.org/10.37983/IJDM.2021.3302
Keywords:
Dentine Hypersensitivity, Tetracalcium phosphate, Calcium hydrogen phosphate, Hydroxyapatite, Octacalcium phosphate
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Original Articles
Statistic

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Tomas Duminis
Queen Mary University of London
https://orcid.org/0000-0002-4959-3199
Saroash Shahid
Institute of Dentistry, Queen Mary University of London

Abstract

Background: Calcium phosphates (CPs) form hydroxyapatite (HA) in physiological solutions. These are commonly used to treat dentine hypersensitivity (DH) as they mimic the mineral composition of the natural tooth.


Aim: The present study aims to characterise the apatite formation ability of a commercially available calcium phosphate TeethmateTM (TM) in physiological-like media.


Materials and Methods: In this study, 4mm (D) x 6mm (L) cylindrical samples of TM were produced and immersed in tris(hydroxymethyl)aminomethane (TRIS) buffer (pH: 7.3) and artificial saliva (AS) (pH: 6.5) for up to 24 hours. This was followed by characterisation of the samples after immersion using 31P magic angle - nuclear magnetic resonance spectroscopy (MAS-NMR), X-ray powder diffraction (XRD) and dentine treated with the material using scanning electron microscopy (SEM).


Results: 31P MAS-NMR and XRD analyses revealed that samples immersed in TRIS buffer solution formed hydroxyapatite within approximately 6 hours of immersion. This change was observed at around 12 hours for samples soaked in AS.  The pH of the immersion media increased with increasing immersion time. SEM analysis showed a transitional phase formation of structures exhibiting plate-like morphology.


Conclusion: This study shows that TM converts to HA in vitro rapidly and provides an effective option for the treatment of dentine hypersensitivity.

Article Details


How to Cite
Duminis, T., & Shahid, S. (2021). In vitro hydroxyapatite formation of a tetracalcium phosphate and anhydrous dicalcium phosphate based dentine desensitiser: TRIS buffer vs artificial saliva. International Journal of Dental Materials, 3(3), 76–83. https://doi.org/10.37983/IJDM.2021.3302
Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

This work is licensed under a Creative Commons Attribution 4.0 International License.

Author Biographies

Tomas Duminis, Queen Mary University of London

Centre for Oral Bioengineering, Institute of Dentistry, Queen Mary University of London, United Kingdom.

Saroash Shahid, Institute of Dentistry, Queen Mary University of London

Centre for Oral Bioengineering, Institute of Dentistry, Queen Mary University of London, United Kingdom.

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