Evaluation of the antimicrobial activity of heat-cure denture base resin materials incorporated with silver nanoparticles


  • Rama Krishna Alla Vishnu Dental College
  • Vineeth Guduri Vishnu Dental College
  • Venkataramana Kandi Prathima Institute of Medical Sciences
  • Raghavendra Swamy KN JSS Dental College & Hospital
  • Ritu Vyas PAHER University
  • Narasimha Rao G Vishnu Dental College




Denture base materials, antifungal agents, nanoparticles,, silver nanoparticles



Poly (Methyl methacrylic acid) based materials are the most widely used for the fabrication of removable complete and partial dentures. Certain microorganisms adhere to the tissue surface of a denture base, especially on palatal region, often leading to Denture stomatitis. Numerous attempts were made to treat the denture stomatitis with various antifungal agents showing variable success rates. This may be attributed to the loss of the drug rapidly into the saliva, inhomogeneous distribution of the drug and the development of resistance to antifungal therapy.


This study was done to evaluate the effect of incorporating various concentrations of silver nanoparticles on the antimicrobial activity of heat-cure denture base resin materials.


Silver nanoparticles were incorporated at various concentrations (0.5, 1.0, 2.0 and 5.0 wt%) into three heat-cure denture base materials. A total of 300 disc-shaped specimens (10 × 2 mm) of heat-cure acrylic resin were made using compression molding technique which comprises 100 specimens with each denture base material. Fifty specimens from each denture base materials were allocated to each microorganism used in the study which comprises into five groups with ten specimens (n=10) for each concentration such as control, 0.5wt%, 1.0wt%, 2.0wt% and 5.0wt% concentrations of silver nanoparticles. Antimicrobial activity of control and modified specimens were evaluated using direct contact method against C albicans, and S Mutans by counting the number of colony-forming units. The data were subjected to One way ANOVA and Tukey HSD tests for statistical analyses.


Significant(P<0.05) differences were observed in the antimicrobial activity against C albicans and S Mutans between the control and modified groups of heat-cure denture base resin materials.


Silver nanoparticles are the favourable materials to incorporate into denture base materials as they exhibit superior antimicrobial activity.

Author Biographies

Rama Krishna Alla, Vishnu Dental College

Assistant Professor, Department of Dental Materials, Vishnu Dental College

Vineeth Guduri, Vishnu Dental College

Assistant Professor, Department of Prosthodontics and Implantology, Vishnu Dental College, Bhimavaram, India – 534202.

Venkataramana Kandi, Prathima Institute of Medical Sciences

Associate Professor of Microbiology, Prathima Institute of Medical Sciences, Karimnagar, Telangana, India.

Raghavendra Swamy KN, JSS Dental College & Hospital

Professor, Department of Prosthodontics, JSS Dental College and Hospital, JSS University, Mysore, India.

Ritu Vyas, PAHER University

Associate Professor, Department of Chemistry, Faculty of Science, PAHER University, Udaipur, India.

Narasimha Rao G, Vishnu Dental College

Assistant Professor, Department of Dental Materials, Vishnu Dental College, Bhimavaram, India – 534202


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