Section Original Articles
Evaluation of the antimicrobial activity of heat-cure denture base resin materials incorporated with silver nanoparticles
Poly (Methyl methacrylic acid) based materials are the most widely used for the fabrication of removable complete and partial dentures. Certain microorganisms adhere to the tissue surface of a denture base, especially on palatal region, often leading to Denture stomatitis. Numerous attempts were made to treat the denture stomatitis with various antifungal agents showing variable success rates. This may be attributed to the loss of the drug rapidly into the saliva, inhomogeneous distribution of the drug and the development of resistance to antifungal therapy.
This study was done to evaluate the effect of incorporating various concentrations of silver nanoparticles on the antimicrobial activity of heat-cure denture base resin materials.
Silver nanoparticles were incorporated at various concentrations (0.5, 1.0, 2.0 and 5.0 wt%) into three heat-cure denture base materials. A total of 300 disc-shaped specimens (10 × 2 mm) of heat-cure acrylic resin were made using compression molding technique which comprises 100 specimens with each denture base material. Fifty specimens from each denture base materials were allocated to each microorganism used in the study which comprises into five groups with ten specimens (n=10) for each concentration such as control, 0.5wt%, 1.0wt%, 2.0wt% and 5.0wt% concentrations of silver nanoparticles. Antimicrobial activity of control and modified specimens were evaluated using direct contact method against C albicans, and S Mutans by counting the number of colony-forming units. The data were subjected to One way ANOVA and Tukey HSD tests for statistical analyses.
Significant(P<0.05) differences were observed in the antimicrobial activity against C albicans and S Mutans between the control and modified groups of heat-cure denture base resin materials.
Silver nanoparticles are the favourable materials to incorporate into denture base materials as they exhibit superior antimicrobial activity.
1. Anusavice KJ. Restorative Resins (in) Denture Base Materials. Saunders, India. 2010.
2. Alla RK. Denture Base Materials (in) Dental Materials Science.
3. Alla RK, Suresh Sajjan MC, Ramaraju AV, Ginjupalli K, Upadhya NP. Influence of fiber reinforcement on the properties of denture base resins. J Biomater Nanobiotech. 2013; 4(1):91-97.
4. Alla RK, Raghavendra Swamy KN, Ritu Vyas, Konakanchi A. Conventional and Contemporary polymers for the fabrication of denture prosthesis: part I – Overview, composition and properties. Int J App Dent Sci. 2015;1(4):82-89.
5. Rama Krishna Alla, K. N. Raghavendra Swamy, Ritu Vyas, Anusha Konakanchi, Vineeth Guduri, Praveen Gadde. Influence of Silver Nanoparticles Incorporation on Flexural Strength of Heat-cure Acrylic Denture Base Resin Materials. Annual Research & Review in Biology. 2017;17(4):1-8.
6. Najla S. Dar-Odeh, Mohammad Al-Beyari, Osama A. Abu-Hammad, The role of antifungal drugs in the management of denture-associated stomatitis, The International Arabic Journal of Antimicrobial Agents 2(1); 2012:1-5.
7. KN Raghavendra Swamy, Rama Krishna Alla, Shammas Mohammed, Anusha Konakanchi. The Role of Antifungal Agents in Treating Denture Stomatitis. Research Journal of Pharmacy and Technology. 2018;11(4):1365-1369.
8. Pachava KR, Shenoy KK, Nadendla LK, Reddy MR, Denture Stomatitis – A Review, Ind J Dent Adv 5(1);2013: 1107-1112.
9. Nagy A, Harrison A, Sabbani S, Munson RS, Dutta PK, Waldman WJ. Silver nanoparticles embedded in zeolite membranes: release of silver ions and mechanism of antibacterial action. Int J Nanomedicine 2011;6:1833–52.
10. Stoimenov P, Klinger R, Marchin G, Klabunde K. Metal Oxide Nanoparticles as Bactericidal Agents. Langmuir 2002;18:6679–86.
11. Nomiya K, Yoshizawa A, Tsukagoshi K, Kasuga NC, Hirakawa S, Watanabe J. Synthesis and structural characterization of silver(I), aluminium (III) and cobalt (II) complexes with 4-isopropyltropolone (hinokitiol) showing noteworthy biological activities. Action of silver(I)-oxygen bonding complexes on the antimicrobial activities. J Inorg Biochem 2004;98:46–60.
12. Marambio-Jones C, Hoek EMV. A review of the antibacterial effects of silver nanomaterials and potential implications for human health and the environment. J Nanoparticle Res 2010;12:1531–51.
13. Sahoo SK, Parveen S, Panda JJ. The present and future of nanotechnology in human health care. Nanomedicine Nanotechnol Biol Med 2007;3:20–31.
14. Rai M, Yadav A, Gade A. Silver nanoparticles as a new generation of antimicrobials. Biotechnol Adv 2009;27:76–83.
15. Kumar R, Münstedt H. Silver ion release from antimicrobial polyamide/silver composites. Biomaterials 2005;26:2081–8.
16. Svanberg M, Mjör IA, Orstavik D. Mutans streptococci in plaque from margins of amalgam, composite, and glass-ionomer restorations. J Dent Res 1990;69:861–4.
17. Bürgers R, Eidt A, Frankenberger R, Rosentritt M, Schweikl H, Handel G, et al. The anti-adherence activity and bactericidal effect of microparticulate silver additives in composite resin materials. Arch Oral Biol 2009;54:595–601.
18. Li LH, Deng JC, Deng HR, Liu ZL, Xin L. Synthesis and characterization of chitosan/ZnO nanoparticle composite membranes. Carbohydr Res 2010;345:994–8.
19. Imazato S. Antibacterial properties of resin composites and dentin bonding systems. Dent Mater 2003;19:449–57.
20. Bundy KJ, Butler MF, Hochman RF. An investigation of the bacteriostatic properties of pure metals. J Biomed Mater Res 1980;14:653–63.
21. Azarsina M, Kasraei S, Yousefi-Mashouf R, Dehghani N, Shirinzad M. The Antibacterial Properties of Composite Resin Containing Nanosilver against Streptococcus mutans and Lactobacillus. Patil SG, editor. J Contemp Dent Pract 2013;14:1014–8.
22. Kasraei S, Sami L, Hendi S, AliKhani M-Y, Rezaei-Soufi L, Khamverdi Z. Antibacterial properties of composite resins incorporating silver and zinc oxide nanoparticles on Streptococcus mutans and Lactobacillus. Restor Dent Endod 2014;39:109–14.
23. Yoshida K, Tanagawa M, Matsumoto S, Yamada T, Atsuta M. Antibacterial activity of resin composites with silver-containing materials. Eur J Oral Sci 1999;107:290–6.
24. Spencer CG, Campbell PM, Buschang PH, Cai J, Honeyman AL. Antimicrobial effects of zinc oxide in an orthodontic bonding agent. Angle Orthod 2009;79:317–22.
25. Acosta-Torres LS, Mendieta I, Nuñez-Anita RE, Cajero-Juárez M, Castaño VM. Cytocompatible antifungal acrylic resin containing silver nanoparticles for dentures. Int J Nanomedicine 2012;7:4777–86.
26. Issa MI, Abdul-Fattah N. Evaluating the effect of silver nanoparticles incorporation on antifungal activity and some properties of soft denture lining material. J Baghdad Coll Dent 2015;27:17–23.
27. Wady AF, Machado AL, Zucolotto V, Zamperini CA, Berni E, Vergani CE. Evaluation of Candida albicans adhesion and biofilm formation on a denture base acrylic resin containing silver nanoparticles: Antifungal activity of silver nanoparticles. J Appl Microbiol 2012;112:1163–72.
28. Kamikawa Y, Hirabayashi D, Nagayama T, Fujisaki J, Hamada T, Sakamoto R, et al. In Vitro Antifungal Activity against Oral Candida Species Using a Denture Base Coated with Silver Nanoparticles. J Nanomater 2014;2014:1–6.
29. Hwang I, Lee J, Hwang JH, Kim K-J, Lee DG. Silver nanoparticles induce apoptotic cell death in Candida albicans through the increase of hydroxyl radicals. FEBS J 2012;279:1327–38.
30. Madeo F, Fröhlich E, Ligr M, Grey M, Sigrist SJ, Wolf DH, et al. Oxygen stress: a regulator of apoptosis in yeast. J Cell Biol 1999;145:757–67.
31. Michalek SM, Hirasawa M, Kiyono H, Ochiai K, McGhee JR. Oral ecology and virulence of Lactobacillus casei and Streptococcus mutans in gnotobiotic rats. Infect Immun 1981;33:690–6.
32. Lara HH, Garza-Treviño EN, Ixtepan-Turrent L, Singh DK. Silver nanoparticles are broad-spectrum bactericidal and virucidal compounds. J Nanobiotechnology 2011;9:30.
33. Ginjupalli K, Shaw T, Tellapragada C, Alla R, Gupta L, Perampalli NU. Does the size matter? Evaluation of effect of incorporation of silver nanoparticles of varying particle size on the antimicrobial activity and properties of irreversible hydrocolloid impression material. Dent Mater 2018;34:e158–65.
34. Azarsina M, Kasraei S, Yousefi-Mashouf R, Dehghani N, Shirinzad M. The Antibacterial Properties of Composite Resin Containing Nanosilver against Streptococcus mutans and Lactobacillus. Patil SG, editor. J Contemp Dent Pract 2013;14:1014–8.
35. Panacek A, Kvítek L, Prucek R, Kolar M, Vecerova R, Pizúrova N, et al. Silver Colloid Nanoparticles:? Synthesis, Characterization, and Their Antibacterial Activity. J Phys Chem B 2006;110:16248–53.
36. Lkhagvajav N, Ya I. Antimicrobial Activity of Colloidal Silver Nanoparticles Prepared By Sol-Gel MethoD. Dig J Nanomater Biostructures 2011;6:149–54.
37. Agnihotri S, Mukherji S, Mukherji S. Size-controlled silver nanoparticles synthesized over the range 5–100 nm using the same protocol and their antibacterial efficacy. RSC Adv 2013;4:3974–83.
38. Ahrari F, Eslami N, Rajabi O, Ghazvini K, Barati S. The antimicrobial sensitivity of Streptococcus mutans and Streptococcus sangius to colloidal solutions of different nanoparticles applied as mouthwashes. Dent Res J 2015;12:44–9.
39. Guzman M, Dille J, Godet S. Synthesis and antibacterial activity of silver nanoparticles against gram-positive and gram-negative bacteria. Nanomedicine Nanotechnol Biol Med 2012;8:37–45.