Effect of incorporating various concentrations of reduced Graphene Oxide (rGO) nanoparticles on the Shore D hardness of 3D-printed denture base resin: An in vitro Study
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Abstract
Background: Three-dimensional (3D) printing is increasingly used for denture base fabrication; however, concerns remain regarding various mechanical properties of 3D-printed denture materials. To address these limitations, nanotechnology has been explored as a promising approach for enhancing material properties. In this context, reduced graphene oxide (rGO), owing to its favourable mechanical properties, has been used for various biomedical applications.
Aim: To evaluate the surface hardness of a 3D-printed denture base resin incorporating different concentrations of rGO.
Materials and methods: A total of 25 standardized disc-shaped specimens were fabricated using a 3D-printing process and divided into five groups with 5 in each. The groups included a control (0 wt%) and four experimental groups containing 0.005 wt%, 0.01 wt%, 0.1 wt%, and 0.25 wt% reduced graphene oxide (rGO), respectively. Specimens were designed using CAD software and fabricated using a DLP-based 3D-printer. Raman spectroscopy was performed to confirm the characteristic peaks of rGO within the resin matrix. Shore D hardness was measured using a manual analogue durometer in accordance with ASTM D2240 standards. Statistical analysis was performed using one-way ANOVA, followed by post hoc tests.
Results: An increase in Shore D hardness was observed in Group 2a (0.005 wt%) and Group 2b (0.01 wt%), both significantly higher than the control, whereas a decrease was noted in Group 2c (0.1 wt%) and Group 2d (0.25 wt%), which were significantly lower than the control. All intergroup comparisons were statistically significant (p < 0.0001).
Conclusion: The findings indicate that the effect of rGO incorporation on shore D hardness is concentration-dependent, but shows an inverse relationship, where lower concentrations result in greater improvement, while higher concentrations lead to reduced performance. The optimal concentrations were found to be 0.005 wt% and 0.01 wt%.
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This work is licensed under a Creative Commons Attribution 4.0 International License.
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1. Alqutaibi AY, Baik A, Almuzaini SA, Farghal AE, Alnazzawi AA, Borzangy S, et al. Polymeric Denture Base Materials: A Review. Polymers (Basel). 202331;15(15):3258. https://doi.org/10.3390/polym15153258.
2. Zafar MS. Prosthodontic Applications of Polymethyl Methacrylate (PMMA): An Update. Polymers (Basel). 2020;12(10):2299. https://doi.org/10.3390/polym12102299.
3. Pillai S, Upadhyay A, Khayambashi P, Farooq I, Sabri H, Tarar M, et al. Dental 3D-Printing: Transferring Art from the Laboratories to the Clinics. Polymers (Basel). 2021;13(1):157. https://doi.org/10.3390/polym13010157.
4. Javaid M, Haleem A. Current status and applications of additive manufacturing in dentistry: A literature-based review. J Oral Biol Craniofac Res. 2019;9(3):179-85. https://doi.org/10.1016/j.jobcr.2019.04.004.
5. Anusavice KJ, Phillips RW, Shen C, Rawls HR. Phillips’ Science of Dental Materials. 13th ed. St. Louis: Elsevier/Saunders; 2013.
6. Song SY, Kim KS, Lee JY, Shin SW. Physical properties and color stability of injection-molded thermoplastic denture base resins. J Adv Prosthodont. 2019;11(1):32-40. https://doi.org/10.4047/jap.2019.11.1.32.
7. Gad MM, Fouda SM, Abualsaud R, Alshahrani FA, Al-Thobity AM, Khan SQ, et al. Strength and Surface Properties of a 3D-Printed Denture Base Polymer. J Prosthodont. 2022;31(5):412-418. https://doi.org/10.1111/jopr.13413.
8. Moosa AA, Kubba F, Raad M, Ramazani SA. Mechanical and thermal properties of graphene nanoplates and functionalized carbon-nanotubes hybrid epoxy nanocomposites. Am J Mater Sci. 2016;6(5):125–34. https://doi.org/10.5923/j.materials.20160605.02
9. Rafiee MA, Rafiee J, Wang Z, Song H, Yu ZZ, Koratkar N. Enhanced mechanical properties of nanocomposites at low graphene content. ACS Nano. 2009;3(12):3884-90. https://doi.org/10.1021/nn9010472.
10. Gad MM, Fouda SM, Al-Harbi FA, Näpänkangas R, Raustia A. PMMA denture base material enhancement: a review of fiber, filler, and nanofiller addition. Int J Nanomed. 2017;12:3801-3812. https://doi.org/10.2147/IJN.S130722.
11. Altarazi A, Haider J, Alhotan A, Silikas N, Devlin H. 3D printed denture base material: The effect of incorporating TiO2 nanoparticles and artificial ageing on the physical and mechanical properties. Dent Mater. 2023;39(12):1122-1136. https://doi.org/10.1016/j.dental.2023.10.005.
12. Atif R, Inam F. Reasons and remedies for the agglomeration of multilayered graphene and carbon nanotubes in polymers. Beilstein J Nanotechnol. 2016;7(1):1174-1196. https://doi.org/10.3762/bjnano.7.109.
13. Zeinedini A, Shokrieh MM. Agglomeration phenomenon in graphene/polymer nanocomposites: reasons, roles, and remedies. Appl Phys Rev. 2024;11(4):041301. https://doi.org/10.1063/5.0223785
14. Sindi AM. Applications of graphene oxide and reduced graphene oxide in advanced dental materials and therapies. J Taibah Univ Med Sci. 2024;19(2):403-421. https://doi.org/10.1016/j.jtumed.2024.02.002.
15. Ferrari AC, Basko DM. Raman spectroscopy as a versatile tool for studying the properties of graphene. Nat Nanotechnol. 2013;8(4):235–246. https://doi.org/10.1038/nnano.2013.46
16. Iravani S. Green synthesis of metal nanoparticles using plants. Green Chem. 2011;13(10):2638–2650. https://doi.org/10.1039/c1gc15386b
17. Spitalsky Z, Tasis D, Papagelis K, Galiotis C. Carbon nanotube-polymer composites: Chemistry, processing, mechanical and electrical properties. Prog Polym Sci. 2010;35(3):357-401.
18. ASTM D2240 Shore Hardness Standard ASTM D2240. Standard Test Method for Rubber Property-Durometer Hardness.
19. Kim D, Shim JS, Lee D, Shin SH, Nam NE, Park KH, et al. Effects of post-curing time on the mechanical and color properties of three-dimensional printed crown and bridge materials. Polymers (Basel). 2020;12(11):2762. https://doi.org/10.3390/polym12112762
20. Tahayeri A, Morgan M, Fugolin AP, Bompolaki D, Athirasala A, Pfeifer CS, et al. 3D printed versus conventionally cured provisional crown and bridge dental materials. Dent Mater. 2018 Feb;34(2):192-200. https://doi.org/10.1016/j.dental.2017.10.003.
21. Singh V, Joung D, Zhai L, Das S, Khondaker SI, Seal S. Graphene based materials: Past, present and future. Prog Mater Sci. 2011;56(8):1178-1271. https://doi.org/10.1016/j.pmatsci.2011.03.003
22. Asar NV, Albayrak H, Korkmaz T, Turkyilmaz I. Influence of various metal oxides on mechanical and physical properties of heat-cured polymethyl methacrylate denture base resins. J Adv Prosthodont. 2013;5(3):241-7.
https://doi.org/10.4047/jap.2013.5.3.241. Epub 2013. Erratum in: J Adv Prosthodont. 2013;5(4):502.
23. Jassim ST, Safi IN, Haider J. Impact of adding cerium zirconium oxide nanofibers in 3D-printed denture base material. J Compos Sci. 2026;10(4):190. https://doi.org/10.3390/jcs10040190
24. Badogu K, Kumar R, Kumar R. Investigations on hardness and surface roughness of 3D printed ABS–ZrO₂ composite structures for post-processing applications. Mater Today Proc. 2023. https://doi.org/10.1016/j.matpr.2023.11.032
25. Sahm BD, Teixeira ABV, Dos Reis AC. Graphene loaded into dental polymers as reinforcement of mechanical properties: A systematic review. Jpn Dent Sci Rev. 2023;59:160-166. https://doi.org/10.1016/j.jdsr.2023.06.003.
26. Dreyer DR, Park S, Bielawski CW, Ruoff RS. The chemistry of graphene oxide. Chem Soc Rev. 2010;39(1):228–240. https://doi.org/10.1039/B917103G
27. Agarwalla SV, Malhotra R, Rosa V. Translucency, hardness and strength parameters of PMMA resin containing graphene-like material for CAD/CAM restorations. J Mech Behav Biomed Mater. 2019;100:103388. https://doi.org/10.1016/j.jmbbm.2019.103388.
28. Punset M, Brizuela A, Pérez-Pevida E, Herrero-Climent M, Manero JM, Gil J. Mechanical Characterization of Dental Prostheses Manufactured with PMMA-Graphene Composites. Materials (Basel). 2022;15(15):5391. https://doi.org/10.3390/ma15155391.
29. Lopez de Armentia Hernandez S, Gimenez Perez R, Del Real JC. Effect of graphene and graphene oxide addition on crosslinking and mechanical properties of photocurable resins for stereolithography. Int J Bioprint. 10(6), 4075. https://doi.org/10.36922/ijb.4075.
30. Stankovich S, Dikin DA, Dommett GH, Kohlhaas KM, Zimney EJ, Stach EA, et al. Graphene-based composite materials. Nature. 2006;442(7100):282-6. https://doi.org/10.1038/nature04969.
31. Ismail Z. Green reduction of graphene oxide by plant extracts: a short review. Ceram Int. 2019;45(18):23857–23868. https://doi.org/10.1016/j.ceramint.2019.08.114
32. Tuinstra F, Koenig JL. Raman spectrum of graphite. J Chem Phys. 1970;53(3):1126–1130. https://doi.org/10.1063/1.1674108
33. Dresselhaus MS, Dresselhaus G, Saito R, Jorio A. Raman spectroscopy of carbon nanotubes. Phys Rep. 2005;409(2):47–99. https://doi.org/10.1016/j.physrep.2004.10.006
34. Bacali C, Badea M, Moldovan M, Sarosi C, Nastase V, Baldea I, et al. The Influence of Graphene in Improvement of Physico-Mechanical Properties in PMMA Denture Base Resins. Materials (Basel). 2019;12(14):2335. https://doi.org/10.3390/ma12142335.
35. Salgado H, Fialho J, Marques M, Vaz M, Figueiral MH, Mesquita P. Mechanical and surface properties of a 3D-printed dental resin reinforced with graphene. Rev Port Estomatol Med Dent Cir Maxilofac. 2023;64(1):12–19. https://doi.org/10.24873/j.rpemd.2023.03.1050