Apical microleakage assessment of teeth obturated with single-cone gutta-percha using two calcium silicate sealers and a resin sealer: an in vitro study.


Main Article Content

Vishal Babu Kolla
R Kalyan Satish
Girija S Sajjan
K Madhu Varma
Ambika Sigadam
Gnana Sindhu Dutta

Abstract

Background: Achieving a fluid-tight seal of the root canal space is the most desirable outcome for the success of endodontic treatment. Root canal sealers are used in combination with core filling materials to fill the irregularities in the root canal, thereby obtaining a three-dimensional seal, which results in the inhibition of bacterial regrowth.


Aim: This study was aimed to compare the apical sealing ability of three root canal sealers AH Plus, Endosequence BC, and Endoseal MTA, using a single cone gutta-percha obturation technique.


Methodology: Forty extracted human single-rooted mandibular premolar teeth were decoronated to a standardized length of 15 mm and instrumented using crown down technique with the ProTaper gold rotary file system to apical file size F3. The roots were randomly allocated into three experimental groups (n=10) and two control groups (n=5). All the samples in experimental groups were obturated with a matched taper single cone: Group 1, Group 2 and Group 3 were obturated using AH Plus, Endosequence BC and Endoseal MTA sealers respectively. Samples were immersed in 1% Methylene blue dye solution for 72 hours, and then the roots were split longitudinally and observed under a stereomicroscope. Apical microleakage was measured from the apex to the most coronal level of dye penetration. The data obtained were subjected to statistical analysis.


Results: Samples in all the groups showed evidence of leakage, except in the negative control group. Group I did not show significant differences with  Group 2 and Group 3 (p = 0.446 and p= 0.147, respectively). Whereas, statistically significant differences were observed between Group 2 and Group 3.


Conclusion:  Endosequence BC showed a superior seal and less microleakage compared to the two other sealers used in this study using a single cone gutta-percha obturation technique.

Article Details


How to Cite
Kolla, V. B., Kalyan Satish, R., Sajjan, G. S., Madhu Varma, K., Sigadam, A., & Dutta, G. S. (2021). Apical microleakage assessment of teeth obturated with single-cone gutta-percha using two calcium silicate sealers and a resin sealer: an in vitro study. International Journal of Dental Materials, 3(4), 100–105. https://doi.org/10.37983/IJDM.2021.3401
Author Biographies

Vishal Babu Kolla, Vishnu Dental College

Postgraduate Student, Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.

R Kalyan Satish, Vishnu Dental College

Professor, Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.

Girija S Sajjan, Vishnu Dental College

Professor, Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.

K Madhu Varma, Vishnu Dental College

Professor, Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.

Ambika Sigadam, Vishnu Dental College

Postgraduate student, Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.

Gnana Sindhu Dutta, Vishnu Dental College

Postgraduate student, Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India.

References

  1. Chandra SS, Shankar P, Indira R. Depth of penetration of four resin sealers into radicular dentinal tubules: a confocal microscopic study. J Endod. 2012;38(10):1412-6. https://doi.org/10.1016/j.joen.2012.05.017
  2. Grossman LI. Endodontic Practice, 10th ed. Philadelphia: Lea and Febiger; 1988:279.
  3. Gutmann JL, Witherspoon DE. Obturation of the cleaned and shaped root canal system. In: Cohen S, Burns R, eds. Pathways of the Pulp, 8th ed. St Louis, MO: CV Mosby; 2004:293–364.
  4. Farea M, Masudi SA, Wan Bakar WZ. Apical microleakage evaluation of system B compared with cold lateral technique: In vitro study. Aust Endod J. 2010;36(2):48-53. https://doi.org/10.1111/j.1747-4477.2009.00187.x
  5. Ingle JI and Taintor JF: Textbook of endodontics. 5th ed
  6. Vallari Jain, Prateeksha Chowdhry, Mamta Kaushik, Roshni Roshni, Neha Mehra. Qualitative and quantitative analysis of apical microleakage of different endodontic sealers. Int J Adv Res. 2019;7(6):476-481. https://doi.org/10.21474/IJAR01/9246
  7. Sadr S, Golmoradizadeh A, Raoof M, Tabanfar MJ. Microleakage of single-cone gutta-percha obturation technique in combination with different types of sealers. Iran Endod J. 2015;10(3):199.
  8. de Miranda Candeiro GT, Correia FC, Duarte MA, Ribeiro-Siqueira DC, Gavini G. Evaluation of radiopacity, pH, release of calcium ions, and flow of a bioceramic root canal sealer. J Endod. 2012;38(6):842-5. https://doi.org/10.1016/j.joen.2012.02.029
  9. Desai S, Chandler N. Calcium hydroxide–based root canal sealers: a review. Journal of Endodontics. 2009;35(4):475-80. https://doi.org/10.1016/j.joen.2008.11.026
  10. Salem AS, Saleh AR, Elmasmari HA. Assessment of Apical Leakage of Bioceramic Endodontic Sealer with Two Obturation Techniques. The Open Dentistry Journal. 2018;12(1): 1162-68. https://doi.org/10.2174/1874210601812011162
  11. Yang D.K., Kim S., Park J.W., Kim E., Shin S.J. Different Setting Conditions Affect Surface Characteristics and Microhardness of Calcium Silicate-Based Sealers. Scanning. 2018;2018:7136345. https://doi.org/10.1155/2018/7136345
  12. Hess D, Solomon E, Spears R, He J. Retreatability of a bioceramic root canal sealing material. J Endod. 2011;37(11):1547-9. https://doi.org/10.1016/j.joen.2011.08.016
  13. Hamid HA, Abdul-kareem J. The effect of smear layer on push-out bond strength to dentin of Bioceramic sealer (In vitro study). J Baghdad Coll Dent. 2013;25(4):5-11. https://doi.org/10.12816/0015057
  14. Remy V, Krishnan V, Job TV, Ravisankar MS, Raj CR, John S. Assessment of Marginal Adaptation and Sealing Ability of Root Canal Sealers: An in vitro Study. J Contemp Dent Pract. 2017;18(12):1130-4. https://doi.org/10.5005/jp-journals-10024-2188
  15. Samiei M, Aghazade M, Farhadi F, Shahveghar N, Torab A, Pakdel SM. Sealing efficacy of single-cone obturation technique with MTA and CEM cement: an in vitro bacterial leakage study. Journal of Dental Research, Dental Clinics, Dental Prospects. 2014;8(2):77.
  16. Whitworth J. Methods of filling root canals: principles and practices. Endod Topics. 2005;12(1):2-4. https://doi.org/10.1111/j.1601-1546.2005.00198.x
  17. Limkangwalmongkol S, Burtscher P, Abbott PV, Sandler AB, Bishop BM. A comparative study of the apical leakage of four root canal sealers and laterally condensed gutta-percha. J Endod. 1991;17(10):495-9. https://doi.org/10.1016/S0099-2399(06)81797-8
  18. Punia SK, Nadig P, Punia V. An in vitro assessment of apical microleakage in root canals obturated with gutta-flow, resilon, thermafil and lateral condensation: A stereomicroscopic study. J Conserv Dent. 2011;14(2):173. https://doi.org/10.4103/0972-0707.82629
  19. Bodrumlu E, Tunga U. Apical leakage of Resilon obturation material. J Contemp Dent Pract. 2006;7(4):45-52. https://doi.org/10.5005/jcdp-7-4-45
  20. Al-Kadhi AM, Al-Ani ZB, Al-Eanizi JA. Comparison of Apical Microleakage of Four Contemporary Endodontic Sealers by Dye Penetration Method. Int Medical J. 2019;26(3):237-40.
  21. Ballullaya SV, Vinay V, Thumu J, Devalla S, Bollu IP, Balla S. Stereomicroscopic dye leakage measurement of six different root canal sealers. J Clin Diagnostic Res. 2017;11(6):ZC65. https://doi.org/10.7860/JCDR/2017/25780.10077
  22. Zhang W, Li Z, Peng B. Assessment of a new root canal sealer's apical sealing ability. Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology. 2009;107(6):e79-82. https://doi.org/10.1016/j.tripleo.2009.02.024
  23. Pawar SS, Pujar MA, Makandar SD. Evaluation of the apical sealing ability of bioceramic sealer, AH plus & epiphany: An in vitro study. J Conserv Dent. 2014;17(6):579. https://doi.org/10.4103/0972-0707.144609
  24. Kim M, Park H, Lee J, Seo H. Microleakage Assessment of a Pozzolan Cement-based Mineral Trioxide Aggregate Root Canal Sealer. J Korean Acad Pediatr Dent. 2017;44(1):20-7. https://doi.org/10.5933/JKAPD.2017.44.1.20
  25. Lim ES, Park YB, Kwon YS, Shon WJ, Lee KW, Min KS. Physical properties and biocompatibility of an injectable calcium-silicate-based root canal sealer: in vitro and in vivo study. BMC oral health. 2015;15(1):1-7. https://doi.org/10.1186/s12903-015-0112-9
  26. Weller RN, Tay KC, Garrett LV, Mai S, Primus CM, Gutmann JL, Pashley DH, Tay FR. Microscopic appearance and apical seal of root canals filled with guttapercha and ProRoot Endo Sealer after immersion in a phosphate-containing fluid. Int Endod J. 2008;41(11):977-86. https://doi.org/10.1111/j.1365-2591.2008.01462.x
  27. HatiboviKofman Š, Raimundo L, Zheng L, Chong L, Friedman M, Andreasen JO. Fracture resistance and histological findings of immature teeth treated with mineral trioxide aggregate. Dent Traumatol. 2008;24(3):272-6. https://doi.org/10.1111/j.1600-9657.2007.00541.x

Most read articles by the same author(s)