Comparative evaluation of sealer penetration depth into radicular dentinal tubules using confocal scanning microscope: an in vitro study

Authors

  • Ambika Sigadam Vishnu Dental College
  • Kalyan Satish R Vishnu Dental College
  • Girija S Sajjan Vishnu Dental College
  • Madhu Varma K Vishnu Dental College
  • Sita Ram Kumar M Vishnu Dental College
  • Praveen D Vishnu Dental College

DOI:

https://doi.org/10.37983/IJDM.2020.2301

Keywords:

Bioceramic sealers, Confocal microscope, Endodontic sealer, Rhodamine B dye

Abstract

Background: Endodontic treatment involves the removal of the vital and necrotic contents of the root canal through chemo-mechanical means followed by obturation of the prepared root canal to prevent the ingress of fluids and avoid bacterial infection or regrowth. Root canal sealers and core filling materials are used together to fill the irregularities in the root. Penetration into the dentinal tubules also results in the inhibition of bacterial regrowth and increases the success of root canal therapy.

Aim: This study aimed to evaluate the penetration depth of various sealers into the dentinal tubules using a confocal microscope.

Materials and methods: A total of 65 specimens were decoronated to standardize the root length of 13mm. Working length was determined, and Biomechanical preparation for all the samples was done with a rotary ProTaper file till F4. Samples were randomly divided into five groups containing 13 teeth in each group based on the sealer used, namely Group 1: Endomethasone (n=13), Group 2: AH-Plus (n=13), Group 3: Roekoseal (n=13), Group 4: MTA Fillapex (n=13), Group 5: Endosequence BC (n=13). All the sealers were labelled with Rhodamine-B dye, and samples were obturated using cold lateral compaction technique. The specimens were sectioned orthogonally at coronal, middle, and apical thirds. All the samples were examined with a Zeiss Pascal Laser Scanning Microscope to examine the sealer penetration depth into the dentinal tubules. The data were subjected to statistical analysis using one- way Analysis of Variance (ANOVA) and Tukey's Honest Significant Difference (HSD) tests.

Results: Endosequence BC showed the highest penetration into dentinal tubules, followed by MTA Fillapex and Roekoseal, AH-Plus, and Endomethasone exhibited the least penetration.

Conclusion: Endosequence BC sealer exhibited maximum penetration. All the groups showed maximum penetration at coronal third, followed by the middle and apical third.

Author Biographies

Kalyan Satish R, Vishnu Dental College

Professor, Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India-534202. Email: rkalyansatish@vdc.edu.in

Girija S Sajjan, Vishnu Dental College

Professor and Head of the Department, Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India-534202. Email: girijasajjan@vdc.edu.in

Madhu Varma K, Vishnu Dental College

Professor, Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India-534202. Email: madhuvarma.k@vdc.edu.in

Sita Ram Kumar M, Vishnu Dental College

Sita Ram Kumar M, MDS, Reader, Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India-534202. Email: sitaramrajubds@gmail.com

Praveen D, Vishnu Dental College

Reader, Department of Conservative Dentistry and Endodontics, Vishnu Dental College, Bhimavaram, Andhra Pradesh, India-534202. Email: praveendalavai@vdc.edu.in

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Published

2020-08-27

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